Brain destructive and degenerative disorders lead not only to the life long decrease, but limit social activity due to the occurrence of the cognitive deficiency.
Experimental and clinical studies showed that brain ischemia follows by occurrence of the active oxygen forms and decrease of the energetic sources. There is a wide spread theory about protective role of the HSP70 in the ischemic damage of the central nervous system due to the activation of the free radicals.
Aim of our work was to study dynamic changes of the oxidative damage markers (nitrotyrosine, oxyhydroxyguanin) in the brain tissue with the parallel study of the HSP70 level.
Materials and methods. Concentration of the HSP70 in the hyppocampus was detected by Western-blot analysis, nitrotyrosine — by immunoassay test, oxyhydroxyguanin — by specrofotometry.
Results. Experimental tests showed that modeling of the brain ischemia leads to the start of the biochemical reactions cascade in the brain tissue with gradual increase of the cell oxidative damage markers — nitrotyrosine, oxyhydroxyguanin. On the other hand, as the answer to the oxidative stress, compensative increase of the citoprotective protein HSP70 was detected. But later HSP70 level much decreases. We suggest that this paradox has connection with weakness of the compensative abilities of the organism. Described changes in the HSP70 protein level in the brain detect perspectiveness and actuality of the new high effective methods of the neuroprotection search between medical drugs that are able to change synthesis and expression of HSP70.