Purpose. A research aim was profound studying the metabolic processes of thiol exchange of urinary bladder detrusor in the observed patients with infravesical obstruction. The obtained data confirmed presence of the condition of oxidative stress in observed patients with infravesical obstruction, which results in violations of thiol status in biological liquids and tissues of urinary bladder and were the reason for the future studying thiol-disulfide state of the examined patients.
Materials and methods. Biochemical studies were conducted using blood, urine and tissue biopsies of the bladder in 59 patients with infravesical obstruction, who underwent preliminary clinical examination. Glutathione-S-tranferase activity was determined in blood plasma, and glutathione-peroxidase activity — in blood hemolisate. The level of thiol and disulfide forms of glutathione was measured in neutralized blood extract. Simultaneously the of thiol and disulfide groups of proteins was determined. The similar studies were carried out in biopsies of the bladder tissue, and also the level of thiol and disulfide groups of proteins in the urine of patients with infravesical obstruction was determined.
Conclusions. Analysing the data of recovery potential of glutathion system in observed patients, we assume that detected metabolic changes are a significant element in the pathogenesis of chronic infravesical obstruction in urological patients.
The results should be regarded as clinical and biochemical basis of necessity to correct glutathion system violations and study its effectiveness under clinical conditions.