Objective. To investigate the blast-transforming lymphocyte reaction in postponed period of corazol kindling in rats and peculiarities of that reaction under conditions of ketogenic diet usage.
Materials and methods. In ficoll-verographin gradient of density the mononuclear cells have been collected, which have been primed with lipopolysaccharide (LPS) E. coli. The index of activation of lymphocytes (IAL) was calculated in accordance to equation: IAL= О/К, where О-blast cells (in %) in samples, which contained both activated by LPS monocytes and phytohemagglutinin (PhHA) stimulated lymphocytes; K-blast cells in samples, which contained intact monocytes and PhHA-stimulated lymphocytes. IAL was determined in blood of corazol-kindled rats in two and four weeks of their being on ketogenic diet (KD) (80% lipids, 3.3% carbohydrates and 16.7% proteins).
Results. Incubation of lymphocytes with LPS during 1,5 h was followed by the increase of blast cells, and their number in control was (59.5±4.7)%, while in kindled rats — 83.7%. IAL was 1.47 and 5.13 correspondently (P<0.05). The number of blast cells in rats, which have been given KD during two weeks was 45.3%, while in kindled rats it was 62.4%. IAL was equal to 0.76 and 1.66 correspondently (P<0.05). Those indices were significantly less when compared with groups of kindled rats, which were not given KD (P<0.05). Maintenance of rats on KD during four weeks was followed the net decrease of blast cells in control and experimental groups up to 41.2 and 48.5%. IAL was also reduced up to 0.7 and 0.95 (P>0.05).
Conclusions. The heightening of LPS-induced blast transformation of lymphocytes is observed in postponed period of kindling and this effect is blocked by KD.