Preadipocytes and differential fat cells have a components for local synthesis of angiotensin II, included chymase, tonin. Violation of secretory function of adipocytes found in chronic hypoxy of fatty tissue and oxidative stress, contributed to the activation of calcium-dependent proteinases — calpains. The changes of calcium concentration directly reflected in the reduction process smooth muscle cells of vascular wall and lead to the hypertension, which at hyperuricemia may contribute to worsening of disease course and development of cardiovascular complications.
The purpose is studying of chymase, tonin and calpains activities at essential hypertension taking into account hyperuricemia.
Hyperuricemia was determined by the presence of uric acid, which was assessed by uricase photometric method using a test-systems of «Cormay», Poland. The activities of calpains, chymase, tonin in blood serum were investigated by high-sensitivity enzymatic method.
The varying degrees of decrease of tonin and calpains activities with increased levels of chymase are revealed in patients with and without hyperuricemia. In patients without hyperuricemia the change of the chymase and tonin activities had a valid character in comparison with the control, and the chymase activity was reduced, and tonin activity had the tend to increase.
The increase of chymase activity, reduction of tonin, calpains activities took place due to vasoconstriction and apoptosis. The lower activity of chymase at hyperuricemia may indicate a violation of its synthesis and release, resulting from increased loss of must cells in adipose tissue. The increased tonin activity indicates to possibility of its synthesis, including the development of vasoconstriction and is caused by increasing concentrations of angiotensinogen in adipose tissue.